Abstract:Acinetobacter baumannii are nosocomial bacteria that are responsible for outbreaks and severe infections in hospitalized patients globally. Its transfer to the environment through feed, soil and water has become a threat to individuals and animals. The major target of this study was the characterization of the resistance determinants of A. baumannii isolates from animal handlers. In total, 19 human samples were collected from animal handlers. The isolates were assesses using phenotypic and genotypic methods, The results showed that 92% of strains presented a multidrug-resistance (MDR), and the highest levels of resistance were to Streptomycin (94%), Cephalosporins (83%) and Ciprofloxacin (83%). The isolates were resistant to more than three antibiotics making it a multidrug resistance organism according to literature and then screened for resistance factor encoding genes using conventional Polymerase Chain Reaction machine.