Abstract:Antibiotic resistance caused by extended spectrum beta-lactamase producing pathogens is ravaging the health care system, affecting the immunocompromised patients more by reason of low immunity. The study investigated the perception of immunocompromised patients towards antibiotics resistance, and characterization of the multidrug-resistant Gram-negative pathogens isolated from their urine. The study was carried out using questionnaire and experimental study, employing patients living with Human Immunodeficiency Virus (HIV), Cancer and Diabetes. Standard microbiological procedures and methods were employed to obtain antibiotics profile, multiple resistance index, multidrug resistant, and ESBL positive pathogens. Data were analyzed using descriptive and inferential statistics, variables with a P-value of <0.05 were considered statistically significant. One hundred and eighty-five patients with consent were recruited with mean age was 44.9 (+14.1). A statistical difference between age, marital status of the patients and their perception to antibiotic resistance was observed (P-value of 0.005, 0.0005). Gram negative bacteria (254) were recovered with 76.8% Multiple Antibiotic Resistance Index (MARI) value above the critical limit (0.2).