Abstract:Curcumin is a lipophilic polyphenol and principal curcuminoid isolated from the rhizomes of the turmeric plant, with diverse pharmacological and biological activities. The molecular interaction of curcumin with Calf thymus DNA (ct-DNA) was studied using spectroscopic approaches under physiological condition. Equilibrium ligand binding experiments were measured in 25 mM Tris-HCl buffer pH 7.4 at 25 0C using FTIR and Shimadzu double beam UV–Visible spectrophotometer (UV-1800) equipped with a 10 mm quartz cell. The study clearly established the interaction between curcumin and ct-DNA with a slight bathochromic shift in the maximum absorption spectra, resulting in perturbation of the hydrophobicity around the polynucleotides bases sequence.