Abstract:Dioscorea species are choice food crops in Africa. However, planting materials are scarce, forcing farmers to rely on mini-setting and planting ware-yams intended for food, which leads to food insecurity and increased hunger. Consequently, this study was designed to develop the most efficient protocols for effective yam breeder seed production through the induction of in vitro rapid shoot proliferation in Kpamyo and Asiedu yam varieties using Conventional Tissue Culture propagation procedures. These white yam varieties were cultured on Murashige and Skoog (MS) 1962 media, fortified with predetermined Plant Growth Regulators: cytokinin KIN and BAP (0.25mg/L-1.0mg/L), augmented with 0.05mg/L of Auxin NAA and IAA, respectively, using one node per each experimental culture, including the control with MS media only. All media recipes were dispensed in two different culture vials, Recycled Mayonnaise Jars (RMJ) and Vented Culture Vials (VCV), to regulate the aeration of in vitro cultures, and each cytokinin was fortified with both auxins singly.